Candida mascoma biography sample
•
WO2011153516A2 - Mold expressing saccharolytic enzymes confirm consolidated bioprocessing using polysaccharide and cellulose - Msn Patents
Info
- Publication number
- WO2011153516A2 WO2011153516A2PCT/US2011/039192US2011039192WWO2011153516A2WO 2011153516 A2WO2011153516 A2WO 2011153516A2US 2011039192 WUS2011039192 WUS 2011039192WWO 2011153516 A2WO2011153516 A2WO 2011153516A2
- Authority
- WO
- WIPO (PCT)
- Prior be off keywords
- beta
- endo
- seq
- glucanase
- host cell
- Prior art date
•
Biofuels pioneer Mascoma LLC and the U.S. Department of Energy's BioEnergy Science Center have developed a revolutionary strain of yeast that could help significantly accelerate the development of biofuels from nonfood plant matter. The approach could provide a pathway to eventual expansion of biofuels production beyond the current output limited to ethanol derived from corn.
C5 FUEL, engineered by researchers at Mascoma and BESC, features fermentation and ethanol yields that set a new standard for conversion of biomass sugars from pretreated corn stover - the non-edible portion of corn crops such as the stalk - converting up to 97 percent of the plant sugars into fuel.
Researchers announced that while conventional yeast leaves more than one-third of the biomass sugars unused in the form of xylose, Mascoma's C5 FUEL efficiently converts this xylose into ethanol, and it accomplishes this feat in less than 48 hours. The finding was presented at the 31st International Fuel Ethanol Workshop in Minneapolis.
'The ability to partner the combined expertise at Mascoma and BESC in engineering microbes to release and convert sugars from lignocellulosic biomass has greatly accelerated the translation of basic research outcomes to a commercial product,' BESC Director Paul Gilna said
•
Abstract
Background
A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellulase preparation (Spezyme CP) and the high ethanol yield Thermoanaerobacterium saccharolyticum strain ALK2. The observed hydrolysis proceeded more slowly than predicted at solids concentrations greater than 50 g/L Avicel. Factors responsible for this inaccuracy were investigated in this study.
Results
Ethanol dramatically reduced cellulase activity in tSSF. At an Avicel concentration of 20 g/L, the addition of ethanol decreased conversion at 96 hours, from 75% in the absence of added ethanol down to 32% with the addition of 34 g/L initial ethanol. This decrease is much greater than expected based on hydrolysis inhibition results in the absence of a fermenting organism. The enhanced effects of ethanol were attributed to the reduced, anaerobic conditions of tSSF, which were shown to inhibit cellulase activity relative to hydrolysis under aerobic conditions. Cellulose hydrolysis in anaerobic conditions was roughly 30% slower than in the presence of air. However, this anaerobic inhibition was reversed by exposing the cellulase enzymes to air.